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Peptide Reconstitution Calculator

Forward mode: how many units to draw. Reverse mode: how much bacteriostatic water to add. Works with U-100, U-50, U-40, and 1 mL syringes.

Prefilled for FOXO4-DRI. View FOXO4-DRI's profile

🧮Reconstitution Calculator

mg

Total amount of peptide in the vial.

mL

Volume of BAC water you'll mix into the vial.

mcg

Draw to this mark

10units

= 0.1 mL on a U-100 syringe

Concentration2.5 mg/mL
Per unit25 mcg
Doses in vial20 doses
Syringe preview10.0 / 100 units
0102030405060708090100

Frequently asked questions

What is the reverse BAC calculator?+

The reverse mode answers the question 'how much bacteriostatic water should I add so each dose lands on a clean tick mark?' You enter the vial size, the dose you want, and the units you'd like to draw — it tells you the exact mL of BAC water to mix in.

How do I calculate units to draw on an insulin syringe?+

Concentration (mg/mL) = vial mg ÷ BAC water mL. Volume (mL) = dose (mg) ÷ concentration. Units on a U-100 syringe = volume × 100. Our calculator handles all of this and lets you pick U-100, U-50, U-40, or a 1 mL tuberculin syringe.

Which syringe should I use for peptide injections?+

U-100 insulin syringes are by far the most common for subcutaneous peptide use because they read in 100 units per mL with very fine markings. U-40 and U-50 syringes are used in some veterinary or specialty contexts. Always match the syringe markings to what your calculator outputs.

Can I use this for GLP-1s like semaglutide and tirzepatide?+

Yes — for compounded vial product. Brand pen products (Ozempic, Wegovy, Mounjaro, Zepbound) use a fixed concentration; for those see our GLP-1 dose escalator. For a compounded multi-dose vial of semaglutide or tirzepatide, this calculator gives you the units per dose.

How accurate is this calculator?+

The math is exact for the inputs you provide. Real-world variability comes from vial overfill (most vials contain 5–15% extra solvent), syringe dead-space, and operator technique. Always verify the math yourself and consult a licensed physician.

Educational use only

This calculator is provided for research and educational purposes. Outputs are derived from the values you enter. Always verify your math and consult a licensed physician before acting on any result.